EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.13.12.19 | D191E | the D191E variant degrades L-Arg and 2-oxoglutarate to pyrroline-5-carboxylate (again detected after reduction to proline and Fmoc derivatization) and succinate nearly stoichiometrically, with only about 5% of the cosubstrate being fragmented to ethylene | Pseudomonas savastanoi pv. phaseolicola |
1.14.20.7 | D191E | the D191E variant degrades L-Arg and 2-oxoglutarateto pyrroline-5-carboxylate (again detected after reduction to proline and Fmoc derivatization) and succinate nearly stoichiometrically, with only about 5% of the cosubstrate being fragmented to ethylene | Pseudomonas savastanoi pv. phaseolicola |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.12.19 | Fe2+ | Fe2+-dependent enzyme | Pseudomonas savastanoi pv. phaseolicola | |
1.14.20.7 | Fe2+ | Fe2+-dependent enzyme | Pseudomonas savastanoi pv. phaseolicola |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.12.19 | 2-oxoglutarate + O2 | Pseudomonas savastanoi pv. phaseolicola | - |
ethene + 3 CO2 + H2O | - |
? | |
1.14.20.7 | L-arginine + 2-oxoglutarate + O2 | Pseudomonas savastanoi pv. phaseolicola | - |
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.12.19 | Pseudomonas savastanoi pv. phaseolicola | P32021 | - |
- |
1.14.20.7 | Pseudomonas savastanoi pv. phaseolicola | P32021 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.12.19 | 2-oxoglutarate + O2 | - |
Pseudomonas savastanoi pv. phaseolicola | ethene + 3 CO2 + H2O | - |
? | |
1.13.12.19 | 2-oxoglutarate + O2 | in the other reaction [EC 1.14.20.7, 2-oxoglutarate/L-arginine monooxygenase/decarboxylase (succinate-forming)] the enzyme catalyses the mono-oxygenation of both 2-oxoglutarate and L-arginine, forming succinate, carbon dioxide and L-hydroxyarginine, which is subsequently cleaved into guanidine and (S)-1-pyrroline-5-carboxylate. An iron(IV)-oxo intermediate initiates L-arginine oxidation but not ethylene production by the 2-oxoglutarate-dependent oxygenase, ethylene-forming enzyme | Pseudomonas savastanoi pv. phaseolicola | ethene + 3 CO2 + H2O | - |
? | |
1.14.20.7 | L-arginine + 2-oxoglutarate + O2 | - |
Pseudomonas savastanoi pv. phaseolicola | succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O | - |
? | |
1.14.20.7 | L-arginine + 2-oxoglutarate + O2 | in the other reaction [EC 1.13.12.19, 2-oxoglutarate dioxygenase (ethene-forming)] the enzyme catalyses the dioxygenation of 2-oxoglutarate forming ethene and three molecules of carbon dioxide. An iron(IV)-oxo intermediate initiates L-arginine oxidation but not ethylene production by the 2-oxoglutarate-dependent oxygenase, ethylene-forming enzyme | Pseudomonas savastanoi pv. phaseolicola | succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.12.19 | EFE | - |
Pseudomonas savastanoi pv. phaseolicola |
1.13.12.19 | ethylene-forming enzyme | - |
Pseudomonas savastanoi pv. phaseolicola |